GB/T 26635-2025 upgrade, list of animal and vegetable oils and fats "vitamin E" testing standards
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GB/T 26635-2025 has been implemented on July 1, 2026, and measures 8 "tocols". CATO has prepared all the standard materials used for testing according to the new national standards.
On December 31, 2025, GB/T 26635-2025 "Animal and vegetable oils and fats - Determination of tocopherol and tocotrienol content - High-performance liquid chromatography method" was released. It has been officially implemented since July 1, 2026, all replacing GB/T 26635-2011, and is equivalent to ISO 9936:2016.
The method determines free α-, β-, γ-, δ-tocopherols and tocotrienols, a total of 8 targets; ester-containing products need to be saponified first; not suitable for milk and dairy products.
01STANDARD
A quick overview of the new national standards
Implemented on 2026.07.01
ISO 9936 equivalent (IDT)
8 target analytes
mobile phase | Volume fraction 3.85% tetrahydrofuran-n-heptane solution |
detector | Fluorescence 295/330 nm (use UV 292 nm when no fluorescence) |
column | 250 mm × 4 mm glycol-based column, or 250 mm × 4.6 mm silica column (approximately 5 μm) |
💡 For samples containing β-tocotrienol, glycol-based columns are preferred: on ordinary silica gel columns, γ-tocopherol and β-tocotrienol will elute at the same time.
02 CHANGES
Changes compared to 2011 version
Major technical changes (3 items)
1scope changed: Explicitly not applicable to milk and milk products.
2Mobile phase changed(See 5.4).
3Reagents: n-hexane is changed to n-heptane (see 9.2.1).
Editorial changes (2 items)
4. The name of the standard is adjusted to "Animal and vegetable oils and fats - Determination of tocopherol and tocotrienol content - High-performance liquid chromatography method" to coordinate with existing standards.
5. Change the paragraphs in 6.1 to list items in order to facilitate reading and use.
03 PRODUCTS
CATO tocol standard list
CATO Jiatu TechnologyCovering all 8 targets of the new national standard (beta-tocopherol is available in racemic form and D form), most are in stock and can be delivered quickly. Click on the item number to view the corresponding product page.
target object | CAS number | Item number (click the corresponding item number to view product details) |
|---|---|---|
alpha-tocopherol | 59-02-9 | |
beta-tocopherol | 148-03-8 | |
D-beta-tocopherol | 16698-35-4 | |
γ-Tocopherol | 54-28-4 | |
delta-tocopherol | 119-13-1 | |
alpha-tocotrienol | 58864-81-6 | |
β-Tocotrienol | 490-23-3 | |
γ-Tocotrienol | 14101-61-2 | |
delta-tocotrienol | 25612-59-3 |
Note: D-β-tocopherol is another stereoconfiguration of β-tocopherol and can be substituted as needed.
Supporting varieties (ester type control, deuterated internal standard and isomer):
use | product | CAS number | Item number |
|---|---|---|---|
Ester type control | DL-alpha-tocopherol acetate | 52225-20-4 | |
Deuterated internal standard | β-Tocopherol-d3 | 936230-75-0 | |
Deuterated internal standard | delta-tocopherol-d4 | 1292815-80-5 | |
Isomers | rac-beta-tocopherol | 48223-98-9 |
A COA is provided with the goods, and HNMR, MS, HPLC/GC spectra are provided; ready-made products will be shipped out on the same day if ordered before 16:00.
The standard information is compiled based on the officially released text of GB/T 26635-2025 (released on December 31, 2025, implemented on July 1, 2026); the delivery date and purity are subject to the real-time display on the CATO official website and the COA accompanying the shipment. The naming of isomers is based on CAS number and structural formula.