Hydantoin Impurity 33 CAS 10040-91-2
| CAS No. | 10040-91-2 |
| Molecular Formula | C12H12N2O4 |
| Molecular Weight | 248.23 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Compared with other compounds in its class, the molecular weight of 248.23 g/mol gives Hydantoin Impurity 33 distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
Hydantoin Impurity 33 (CAS 10040-91-2) is a certified pharmaceutical impurity reference standard with the molecular formula C12H12N2O4 and a molecular weight of 248.23 g/mol.
The molecular framework of Hydantoin Impurity 33 (C12H12N2O4) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
As a certified reference material (CRM), Hydantoin Impurity 33 meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C12H12N2O4 |
| Molecular Weight | 248.23 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | >95% |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 8.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1959 |
| Nitrogen Atoms | 2 |
Related Hydantoin Standards
- Hydantoin 1-Benzyl 5-Ethoxy (CAS 65855-02-9)
- Hydantoin Impurity 31 (CAS 110932-42-8)
- Hydantoin Impurity 5 (CAS 1433954-32-5)
- Hydantoin 5-Cyclobutyl 5-Phenyl (CAS 125650-44-4)
- Hydantoin Impurity 1 (CAS 6859-11-6)
- Hydantoin Impurity 3 (CAS 4224-00-4)
- Hydantoin 1-Dodecyl (CAS 85391-28-2)
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Recommended Standards
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- Ethyl (2R,3R)-3-phenyloxirane-2-carboxylate (CAS 126060-73-9)
- Bendamustine Impurity 7 (CAS 1138238-08-0)
- 1-(2-Hydroxy-4,6-dimethoxyphenyl)-2-(4-methoxyphenyl)ethan-1-one (CAS 39604-68-7)
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