(3α,5 β,7α)-3,7-Dihydroxy-12-oxocholan-24-oic Acid Methyl Ester CAS 10538-64-4
| CAS No. | 10538-64-4 |
| Molecular Formula | C25H40O5 |
| Molecular Weight | 420.58 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
(3α,5 β,7α)-3,7-Dihydroxy-12-oxocholan-24-oic Acid Methyl Ester (CAS 10538-64-4) is a high-purity analytical reference standard with the molecular formula C25H40O5 and a molecular weight of 420.58 g/mol.
Owing to a steroid core, a hydroxyl group, an ether linkage, (3α,5 β,7α)-3,7-Dihydroxy-12-oxocholan-24-oic Acid Methyl Ester is typically off-white to pale yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 420.58 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
As a certified reference material (CRM), (3α,5 β,7α)-3,7-Dihydroxy-12-oxocholan-24-oic Acid Methyl Ester meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C25H40O5 |
| Molecular Weight | 420.58 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, acetonitrile, DMSO, water (slightly soluble) |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1960 |
Related Dihydroxy Standards
- (3α,5β,12α)-3,12-Dihydroxy-7-oxocholan-24-oic acid methyl ester (CAS 10538-65-5)
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- (3β,22S)-Dihydroxy Cholesterol (CAS 26649-05-8)
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Recommended Standards
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