Cholic Acid Impurity N1 (3,7,22-Trihydroxyl-Cholanic Acid) CAS 108266-90-6

Description

Compared with other compounds in its class, the molecular weight of 408.57 g/mol gives Cholic Acid Impurity N1 (3,7,22-Trihydroxyl-Cholanic Acid) distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

As a process- or degradation-related impurity, Cholic Acid Impurity N1 (3,7,22-Trihydroxyl-Cholanic Acid) (CAS 108266-90-6, C24H40O5) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.

Owing to a steroid core, a hydroxyl group, an ether linkage, Cholic Acid Impurity N1 (3,7,22-Trihydroxyl-Cholanic Acid) is typically off-white to pale yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 408.57 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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