(E)-4-[4-(4-{[(E)-4-hydroxy-4-oxo-2-butenoyl]amino}phenoxy)anilino]-4-oxo-2-butenoic acid CAS 1164482-89-6

Purity 97%+In StockPharmaceutical Standards
CAS No.1164482-89-6
Molecular FormulaC20H16N2O7
Molecular Weight396.35 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

(E)-4-[4-(4-{[(E)-4-hydroxy-4-oxo-2-butenoyl]amino}phenoxy)anilino]-4-oxo-2-butenoic acid CAS 1164482-89-6 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 396.35 g/mol gives (E)-4-[4-(4-{[(E)-4-hydroxy-4-oxo-2-butenoyl]amino}phenoxy)anilino]-4-oxo-2-butenoic acid distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

(E)-4-[4-(4-{[(E)-4-hydroxy-4-oxo-2-butenoyl]amino}phenoxy)anilino]-4-oxo-2-butenoic acid (CAS 1164482-89-6) is a high-purity analytical reference standard with the molecular formula C20H16N2O7 and a molecular weight of 396.35 g/mol.

The molecular framework of (E)-4-[4-(4-{[(E)-4-hydroxy-4-oxo-2-butenoyl]amino}phenoxy)anilino]-4-oxo-2-butenoic acid (C20H16N2O7) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), (E)-4-[4-(4-{[(E)-4-hydroxy-4-oxo-2-butenoyl]amino}phenoxy)anilino]-4-oxo-2-butenoic acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC20H16N2O7
Molecular Weight396.35 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)14.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1989
Nitrogen Atoms2

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