| CAS No. | 1173-32-6 |
| Molecular Formula | C25H40O3 |
| Molecular Weight | 388.58 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Methyl-3-keto-5β-cholan-24-oate (CAS 1173-32-6), molecular formula C25H40O3 and molecular weight 388.58 g/mol, is a CATO reference standard with documented purity and traceability.
Based on its molecular formula C25H40O3, Methyl-3-keto-5β-cholan-24-oate contains a steroid core, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 388.58 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures Methyl-3-keto-5β-cholan-24-oate under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
| Molecular Formula | C25H40O3 |
| Molecular Weight | 388.58 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |