5-Methyl Cytosine-13C,15N2 Hydrochloride CAS 1246815-59-7

Description

The occurrence of 5-Methyl Cytosine-13C,15N2 Hydrochloride is closely related to an amide group, a hydroxyl group, an ether linkage, chlorine substitution in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

5-Methyl Cytosine-13C,15N2 Hydrochloride (CAS 1246815-59-7), molecular formula C4[13C]H7N[15N]2O HCl and molecular weight 147.58 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 5-Methyl Cytosine-13C,15N2 Hydrochloride (C4[13C]H7N[15N]2O HCl) is characterized by an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 5-Methyl Cytosine-13C,15N2 Hydrochloride meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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