5-Methoxy-1H-pyrazolo[3,4-b]pyridine CAS 1256804-26-8

Description

Compared with other compounds in its class, the molecular weight of 149.15 g/mol gives 5-Methoxy-1H-pyrazolo[3,4-b]pyridine distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

5-Methoxy-1H-pyrazolo[3,4-b]pyridine (CAS 1256804-26-8), molecular formula C7H7N3O and molecular weight 149.15 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 5-Methoxy-1H-pyrazolo[3,4-b]pyridine (C7H7N3O) is characterized by an amide group, a hydroxyl group, an ether linkage, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 5-Methoxy-1H-pyrazolo[3,4-b]pyridine meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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