5,6-Dibromo-3',6'-dihydroxy-3h-spiro[isobenzofuran-1,9'-xanthen]-3-one CAS 13232-65-0

Description

5,6-Dibromo-3',6'-dihydroxy-3h-spiro[isobenzofuran-1,9'-xanthen]-3-one (CAS 13232-65-0), molecular formula C20H10Br2O5 and molecular weight 490.10 g/mol, is a CATO reference standard with documented purity and traceability.

Owing to a flavonoid core, a hydroxyl group, an ether linkage, bromine substitution, 5,6-Dibromo-3',6'-dihydroxy-3h-spiro[isobenzofuran-1,9'-xanthen]-3-one is typically pale yellow to yellow crystalline powder. Its solid-state physicochemical attributes — including a molecular weight of 490.10 g/mol — guide the selection of appropriate sample preparation and analytical techniques. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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