| CAS No. | 137-44-0 |
| Molecular Formula | C3H4N2O |
| Molecular Weight | 84.08 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
1H-Pyrazol-5(4H)-one (CAS 137-44-0), molecular formula C3H4N2O and molecular weight 84.08 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of 1H-Pyrazol-5(4H)-one (C3H4N2O) is characterized by an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C3H4N2O |
| Molecular Weight | 84.08 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 3.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1957 |
| Nitrogen Atoms | 2 |