N-(4,6-Dichloro-5-nitropyrimidin-2-yl)formamide CAS 139710-32-0

Description

The occurrence of N-(4,6-Dichloro-5-nitropyrimidin-2-yl)formamide is closely related to an amide group, a carboxylic acid group, a hydroxyl group, an ether linkage, chlorine substitution in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

N-(4,6-Dichloro-5-nitropyrimidin-2-yl)formamide (CAS 139710-32-0) is a high-purity analytical reference standard with the molecular formula C5H2Cl2N4O3 and a molecular weight of 237.00 g/mol.

The molecular framework of N-(4,6-Dichloro-5-nitropyrimidin-2-yl)formamide (C5H2Cl2N4O3) is characterized by an amide group, a carboxylic acid group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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