7-Methyl-2-(4-methylphenyl)-5-nitro-1,3-benzoxazole CAS 1421241-45-3

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 7-Methyl-2-(4-methylphenyl)-5-nitro-1,3-benzoxazole.

7-Methyl-2-(4-methylphenyl)-5-nitro-1,3-benzoxazole (CAS 1421241-45-3), molecular formula C15H12N2O3 and molecular weight 268.27 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 7-Methyl-2-(4-methylphenyl)-5-nitro-1,3-benzoxazole (C15H12N2O3) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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