2-Amino-1-ethyl-4-hydroxy-5-oxo-4,5-dihydro-1H-imidazole-4-carboxylic acid CAS 144776-84-1

Purity 97%+In StockPharmaceutical Standards
CAS No.144776-84-1
Molecular FormulaC6H9N3O4
Molecular Weight187.15 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

2-Amino-1-ethyl-4-hydroxy-5-oxo-4,5-dihydro-1H-imidazole-4-carboxylic acid CAS 144776-84-1 chemical structure

Description

The occurrence of 2-Amino-1-ethyl-4-hydroxy-5-oxo-4,5-dihydro-1H-imidazole-4-carboxylic acid is closely related to an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

2-Amino-1-ethyl-4-hydroxy-5-oxo-4,5-dihydro-1H-imidazole-4-carboxylic acid (CAS 144776-84-1) is a high-purity analytical reference standard with the molecular formula C6H9N3O4 and a molecular weight of 187.15 g/mol.

The molecular framework of 2-Amino-1-ethyl-4-hydroxy-5-oxo-4,5-dihydro-1H-imidazole-4-carboxylic acid (C6H9N3O4) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 2-Amino-1-ethyl-4-hydroxy-5-oxo-4,5-dihydro-1H-imidazole-4-carboxylic acid meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC6H9N3O4
Molecular Weight187.15 g/mol
AppearanceOff-white to pale yellow crystalline powder. (faint amine-like odor)
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry
Solubility (reference)Soluble in methanol, ethanol, DMSO, water
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)4.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1998
Nitrogen Atoms3

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