3-Amino-4-hydroxycyclobut-3-ene-1,2-dione CAS 150583-44-1

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 3-Amino-4-hydroxycyclobut-3-ene-1,2-dione.

3-Amino-4-hydroxycyclobut-3-ene-1,2-dione (CAS 150583-44-1) is a high-purity analytical reference standard with the molecular formula C4H3NO3 and a molecular weight of 113.07 g/mol.

The molecular framework of 3-Amino-4-hydroxycyclobut-3-ene-1,2-dione (C4H3NO3) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 3-Amino-4-hydroxycyclobut-3-ene-1,2-dione meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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