Ethyl (E)-2-((1H-indol-3-yl)methylene)hydrazine-1-carboxylate CAS 15641-27-7

Description

The occurrence of Ethyl (E)-2-((1H-indol-3-yl)methylene)hydrazine-1-carboxylate is closely related to an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

Ethyl (E)-2-((1H-indol-3-yl)methylene)hydrazine-1-carboxylate (CAS 15641-27-7), molecular formula C12H13N3O2 and molecular weight 231.25 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of Ethyl (E)-2-((1H-indol-3-yl)methylene)hydrazine-1-carboxylate (C12H13N3O2) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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