| CAS No. | 186527-30-0 |
| Molecular Formula | C12H21N3O2 |
| Molecular Weight | 239.31 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
2-Nitro-3-methyl-9H-pyrido[2,3-b]indole (CAS 186527-30-0) is a high-purity analytical reference standard with the molecular formula C12H21N3O2 and a molecular weight of 239.31 g/mol.
The molecular framework of 2-Nitro-3-methyl-9H-pyrido[2,3-b]indole (C12H21N3O2) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO manufactures 2-Nitro-3-methyl-9H-pyrido[2,3-b]indole under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
| Molecular Formula | C12H21N3O2 |
| Molecular Weight | 239.31 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 4.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2010 |
| Nitrogen Atoms | 3 |