2-[(2,2-Dimethylpropyl)({[(9H-fluoren-9-yl)methoxy]carbonyl})amino]acetic acid CAS 1935967-49-9

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

2-[(2,2-Dimethylpropyl)({[(9H-fluoren-9-yl)methoxy]carbonyl})amino]acetic acid (CAS 1935967-49-9) is a high-purity analytical reference standard with the molecular formula C22H25NO4 and a molecular weight of 367.44 g/mol.

The molecular framework of 2-[(2,2-Dimethylpropyl)({[(9H-fluoren-9-yl)methoxy]carbonyl})amino]acetic acid (C22H25NO4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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