2044-85-1 Diacetyldichlorofluorescein Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro- DCFDA Dcfh-DA 2',7'-Dichlorofluorescein diacetate 2',7'-Dichlorofluorescin diacetate Leuco-dcf diacetate Leukodiacetyl-2,7-dichlorofluorescein 3',6'-Bis(acetyloxy)-2',7'-dichlorospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one CAS 2044-85-1

Purity 97%+In StockPharmaceutical Standards
CAS No.2044-85-1
Molecular FormulaC24H32Cl2O7
Molecular Weight503.41 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

2044-85-1 Diacetyldichlorofluorescein Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro- DCFDA Dcfh-DA 2',7'-Dichlorofluorescein diacetate 2',7'-Dichlorofluorescin diacetate Leuco-dcf diacetate Leukodiacetyl-2,7-dichlorofluorescein 3',6'-Bis(acetyloxy)-2',7'-dichlorospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one CAS 2044-85-1 chemical structure

Description

2044-85-1 Diacetyldichlorofluorescein Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro- DCFDA Dcfh-DA 2',7'-Dichlorofluorescein diacetate 2',7'-Dichlorofluorescin diacetate Leuco-dcf diacetate Leukodiacetyl-2,7-dichlorofluorescein 3',6'-Bis(acetyloxy)-2',7'-dichlorospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one (CAS 2044-85-1) is a high-purity analytical reference standard with the molecular formula C24H32Cl2O7 and a molecular weight of 503.41 g/mol.

The molecular framework of 2044-85-1 Diacetyldichlorofluorescein Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro- DCFDA Dcfh-DA 2',7'-Dichlorofluorescein diacetate 2',7'-Dichlorofluorescin diacetate Leuco-dcf diacetate Leukodiacetyl-2,7-dichlorofluorescein 3',6'-Bis(acetyloxy)-2',7'-dichlorospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one (C24H32Cl2O7) is characterized by a flavonoid core, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the polarity and ionization state of its functional groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 2044-85-1 Diacetyldichlorofluorescein Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-bis(acetyloxy)-2',7'-dichloro- DCFDA Dcfh-DA 2',7'-Dichlorofluorescein diacetate 2',7'-Dichlorofluorescin diacetate Leuco-dcf diacetate Leukodiacetyl-2,7-dichlorofluorescein 3',6'-Bis(acetyloxy)-2',7'-dichlorospiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC24H32Cl2O7
Molecular Weight503.41 g/mol
AppearancePale yellow to yellow crystalline powder. (slight halide-like odor, low volatility)
Purity97%+
StorageStore at room temperature, protect from light, keep sealed and dry
Solubility (reference)Soluble in methanol, acetonitrile, DMSO, water (slightly soluble)
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Contains ester bond; hydrolyzes under strong alkaline conditions
Degree of Unsaturation (DBE)8.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1957
Halogen ContentCl-containing

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