(25R)-3α,7α-dihydroxy-5 β-cholestan-26-oic acid CAS 23740-16-1
| CAS No. | 23740-16-1 |
| Molecular Formula | C27H46O4 |
| Molecular Weight | 434.65 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The molecular framework of (25R)-3α,7α-dihydroxy-5 β-cholestan-26-oic acid (C27H46O4) is characterized by a steroid core, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.
(25R)-3α,7α-dihydroxy-5 β-cholestan-26-oic acid (CAS 23740-16-1), molecular formula C27H46O4 and molecular weight 434.65 g/mol, is a CATO reference standard with documented purity and traceability.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C27H46O4 |
| Molecular Weight | 434.65 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at room temperature, keep sealed and dry |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate, DMSO |
| Stability | May oxidize slowly on prolonged air exposure |
| Degree of Unsaturation (DBE) | 5.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1963 |
Related R Standards
- 1,24-Dihydroxy-25-Fluorovitamin D3 (CAS 71699-09-7)
- 1α,25-Dihydroxy-3-epi-vitamin D2-D6 (CAS 1202355-87-0)
- 1α,25-Dihydroxy Vitamin D2-d6 (CAS 216244-04-1)
- 5,7-Dihydroxy 3,3',4',5',6,8-hexamethoxyflavone (CAS 96887-18-2)
- 3β,7α-dihydroxy-5-cholestenoic acid (CAS 115538-84-6)
- 1,6-Dihydroxy-3-methyl-8-(((2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)anthracene-9,10-dione (CAS 52731-38-1)
- 24,25-Dihydroxy vitamin D2-d (CAS 118584-50-2)
- 1,25-Dihydroxy Vitamin D2-d (CAS 1261254-46-9)
Recommended Standards
- 3α,7α-Dihydroxycoprostanic Acid (CAS 17974-66-2)
- CAS 2260669-23-4 (CAS 2260669-23-4)
- (3R,5S,6R,7R,8S,9S,10S,13R,14S,17R)-6-Ethyl-7-hydroxy-17-((R)-4-hydroxybutan-2-yl)-10,13-dimethylhexadecahydro-1H-cyclopenta[a]phenanthren-3-yl acetate (CAS 1605309-13-4)
- 2,5-dichloro-N'-[3-chloro-5-(trifluoromethyl)-2-pyridinyl]-N'-methylbenzenesulfonohydrazide 339276-23-2 KS-000037LN (CAS 339276-23-2)
- 2-Methoxy-6-nitropyridine (CAS 1246566-53-9)
- 6-Amino-2,4-dimethyl-2H-benzo[b][1,4]oxazin-3(4H)-one (CAS 70488-69-6)
- 1-(3-(4-Benzhydrylpiperazin-1-yl)propyl)-1,3-dihydro-2H-benzo[d]imidazol-2-one dihydrochloride (CAS 65215-19-2)
- methyl 4-fluoro-3-methoxybenzoate (CAS 74385-37-8)
- 3-(Acetamidomethyl)benzoic acid (CAS 777-69-5)
- 5-Bromo-6-morpholinonicotinic acid (CAS 1216637-77-2)
- 1-(1-Isopentyl-2-oxo-1,2,3,4-tetrahydroquinolin-6-yl)-3-(thiophen-2-yl)urea (CAS 1171925-70-4)
- 2-(Aminooxy)propane hydrochloride (CAS 4490-81-7)
- (1R,1'S,3'R/1R,1'R,3'S)-L-054,264 N-[(1R)-2-[[[(1S*,3R*)-3-(Aminomethyl)cyclohexyl]methyl]amino]-1-(1H-indol-3-ylmethyl)-2-oxoethyl]spiro[1H-indene-1,4'-piperidine]-1'-carboxamide 208706-12-1 (CAS 208706-12-1)
- Semaglutide impurity 36 (CAS CATO-C4X-222636)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
- Worldwide shipping with full export documentation (HS code guidance available)
- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com