| CAS No. | 2435-59-8 |
| Molecular Formula | C10H8N4O4 |
| Molecular Weight | 248.19 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 5,5'-Diamino-2H,2'H-[3,3'-bipyridinylidene]-2,2',6,6'(1H,1'H)-tetraone.
5,5'-Diamino-2H,2'H-[3,3'-bipyridinylidene]-2,2',6,6'(1H,1'H)-tetraone (CAS 2435-59-8) is a high-purity analytical reference standard with the molecular formula C10H8N4O4 and a molecular weight of 248.19 g/mol.
5,5'-Diamino-2H,2'H-[3,3'-bipyridinylidene]-2,2',6,6'(1H,1'H)-tetraone (C10H8N4O4) contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C10H8N4O4 |
| Molecular Weight | 248.19 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (faint amine-like odor) |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 9.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |
| Nitrogen Atoms | 4 |