1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil CAS 25137-84-2

Purity 97%+In StockPharmaceutical Standards
CAS No.25137-84-2
Molecular FormulaC25H22Cl2N2O7
Molecular Weight533.36 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil CAS 25137-84-2 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 533.36 g/mol gives 1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil (CAS 25137-84-2), molecular formula C25H22Cl2N2O7 and molecular weight 533.36 g/mol, is a CATO reference standard with documented purity and traceability.

1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil (C25H22Cl2N2O7) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC25H22Cl2N2O7
Molecular Weight533.36 g/mol
AppearancePale yellow to yellow crystalline powder. (slight halide-like odor, low volatility)
Purity97%+
StorageStore in a cool place (≤25°C), protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions
Degree of Unsaturation (DBE)15.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)1964
Halogen ContentCl-containing
Nitrogen Atoms2

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