| CAS No. | 25137-84-2 |
| Molecular Formula | C25H22Cl2N2O7 |
| Molecular Weight | 533.36 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 533.36 g/mol gives 1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil (CAS 25137-84-2), molecular formula C25H22Cl2N2O7 and molecular weight 533.36 g/mol, is a CATO reference standard with documented purity and traceability.
1-(3,5-Di-O-p-chlorobenzoyl-2-deoxy-β-D-ribofuranosyl)-5-ethyluracil (C25H22Cl2N2O7) contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. It is notably hygroscopic, halogen substitution raises its density and boiling point, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C25H22Cl2N2O7 |
| Molecular Weight | 533.36 g/mol |
| Appearance | Pale yellow to yellow crystalline powder. (slight halide-like odor, low volatility) |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 15.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1964 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 2 |