N-(3-Nitrophenyl)-3-oxobutanamide CAS 25233-49-2

Description

Compared with other compounds in its class, the molecular weight of 222.20 g/mol gives N-(3-Nitrophenyl)-3-oxobutanamide distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

N-(3-Nitrophenyl)-3-oxobutanamide (CAS 25233-49-2) is a high-purity analytical reference standard with the molecular formula C10H10N2O4 and a molecular weight of 222.20 g/mol.

Based on its molecular formula C10H10N2O4, N-(3-Nitrophenyl)-3-oxobutanamide contains an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 222.20 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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