4H-Benzo[d][1,3]oxazine CAS 253-93-0

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 4H-Benzo[d][1,3]oxazine.

4H-Benzo[d][1,3]oxazine (CAS 253-93-0), molecular formula C8H7NO and molecular weight 133.15 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 4H-Benzo[d][1,3]oxazine (C8H7NO) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 4H-Benzo[d][1,3]oxazine meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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