Folic Acid Impurity 65 CAS 25663-25-6
| CAS No. | 25663-25-6 |
| Molecular Formula | C19H18N6O7 |
| Molecular Weight | 442.38 g/mol |
| Category | Pharmacopoeial Impurity Standards / EP Impurities |
| Availability | In Stock |
Chemical Structure
Description
Based on its molecular formula C19H18N6O7, Folic Acid Impurity 65 contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 442.38 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).
As a process- or degradation-related impurity, Folic Acid Impurity 65 (CAS 25663-25-6, C19H18N6O7) is essential for impurity profiling, related-substances testing, and forced-degradation studies in pharmaceutical development.
Recommended for quantitative analysis by HPLC, GC, LC-MS/MS, or GC-MS as an external calibration standard; it can also be used for spiked-recovery experiments, LOD/LOQ determination, and LC-MS/MS multiple-reaction-monitoring (MRM) calibration.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C19H18N6O7 |
| Molecular Weight | 442.38 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 14.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1964 |
| Nitrogen Atoms | 6 |
Related Folic Standards
Recommended Standards
- N-(3,5-Dichlorophenyl)-2-((3-ethyl-5,6-dimethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-2-yl)thio)acetamide (CAS 577763-67-8)
- Vilanterol Impurity 72 (CAS CATO-C4X-193572)
- N-(3,4-Dichlorophenyl)-2-((3-ethyl-5,6-dimethyl-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-2-yl)thio)acetamide (CAS 618427-83-1)
- N-Nitroso-N-Desmethyl Cariprazine (CAS CATO-C4X-24086)
- Prednisolone impurity 59 (CAS CATO-C4X-173759)
- Brivaracetam Impurity 178 (CAS CATO-C4X-2007178)
- Mebeverine Impurity 17 (CAS CATO-C4X-168717)
- (2-Methylbutyl)(2-{[1,2,4]triazolo[4,3-a]pyridin-3-yl}ethyl)amine dihydrochloride (CAS 1258640-34-4)
- (1-(3-Bromo-4-methoxybenzyl)cyclopropyl)methanol (CAS 1485815-16-4)
- 6-Iodopicolinohydrazide (CAS 851102-43-7)
- Flumetasone Impurity 7 (CAS 61633-73-6)
- 3-(3-Fluorophenyl)acrylaldehyde (CAS 42291-83-8)
- (R)-Lofexidine (CAS 81447-78-1)
- Ephrin-A2-Selective YSA-Peptide (CAS 532441-10-4)
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