6-(2-Benzylidenehydrazinyl)-1,3-dimethylpyrimidine-2,4(1H,3H)-dione CAS 25774-97-4

Description

6-(2-Benzylidenehydrazinyl)-1,3-dimethylpyrimidine-2,4(1H,3H)-dione (CAS 25774-97-4), molecular formula C13H14N4O2 and molecular weight 258.28 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 6-(2-Benzylidenehydrazinyl)-1,3-dimethylpyrimidine-2,4(1H,3H)-dione (C13H14N4O2) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 6-(2-Benzylidenehydrazinyl)-1,3-dimethylpyrimidine-2,4(1H,3H)-dione meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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