2H-Pyrazolo[3,4-d]pyrimidine CAS 271-78-3
| CAS No. | 271-78-3 |
| Molecular Formula | C5H4N4 |
| Molecular Weight | 120.11 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
2H-Pyrazolo[3,4-d]pyrimidine (CAS 271-78-3), molecular formula C5H4N4 and molecular weight 120.11 g/mol, is a CATO reference standard with documented purity and traceability.
The molecular framework of 2H-Pyrazolo[3,4-d]pyrimidine (C5H4N4) is characterized by a nitrile group. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
Recommended for analytical method development and validation; each product is supplied with a full Certificate of Analysis (COA) ensuring ISO 17034 metrological traceability.
As a certified reference material (CRM), 2H-Pyrazolo[3,4-d]pyrimidine meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C5H4N4 |
| Molecular Weight | 120.11 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| CAS Registry Year (est.) | 1957 |
| Nitrogen Atoms | 4 |
Related H Standards
- 1H-Pyrazolo[3,4-b]pyrazine (CAS 272-60-6)
- 1H-Pyrazolo[3,4-c]pyridazine (CAS 271-75-0)
- 1H-Pyrazolo[3,4-d]pyridazine (CAS 271-55-6)
- 2H-Pyrazolo[3,4-b]pyrazine (CAS 24977-38-6)
- 2H-Pyrazolo[4,3-d]pyrimidine (CAS 272-55-9)
- 3H-Pyrazolo[3,4-d]pyrimidine (CAS 271-79-4)
- 4H-Pyrazolo[3,4-d]pyrimidine (CAS 271-76-1)
- 7H-Pyrazolo[3,4-b]pyrazine (CAS 399-62-2)
Recommended Standards
- 2H-[1,2,3]Triazolo[4,5-b]pyridine (CAS 273-33-6)
- 2-Hydroxy-3-cyanopyridine (CAS 20577-27-9)
- 2-Formylpyrrole-3-carbonitrile (CAS 56164-43-3)
- 2-Hydroxyethyl hydrazinecarboxylate (CAS 4341-20-2)
- Pyraclostrobin Impurity 4 (CAS 151827-82-6)
- 6-(4-Bromophenyl)-3-(ethylthio)-[1,2,4]triazolo[4,3-b]pyridazine (CAS 894051-99-1)
- Ethyl 5-(chlorosulfonyl)-2-iodobenzoate (CAS 1155911-38-8)
- 5-(7-Methoxy-1-benzofuran-2-yl)-1,3,4-oxadiazol-2-amine (CAS 1105193-69-8)
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