2H-Pyrazolo[4,3-b]pyridine CAS 272-51-5

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 2H-Pyrazolo[4,3-b]pyridine.

2H-Pyrazolo[4,3-b]pyridine (CAS 272-51-5) is a high-purity analytical reference standard with the molecular formula C6H5N3 and a molecular weight of 119.12 g/mol.

The molecular framework of 2H-Pyrazolo[4,3-b]pyridine (C6H5N3) is characterized by a nitrile group, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 2H-Pyrazolo[4,3-b]pyridine meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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