1-Phenyl-1H-indole-2,3-dione 3-[N-(3,4-dichlorophenyl)hydrazone] CAS 303984-74-9

Description

Compared with other compounds in its class, the molecular weight of 382.24 g/mol gives 1-Phenyl-1H-indole-2,3-dione 3-[N-(3,4-dichlorophenyl)hydrazone] distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

1-Phenyl-1H-indole-2,3-dione 3-[N-(3,4-dichlorophenyl)hydrazone] (CAS 303984-74-9) is a high-purity analytical reference standard with the molecular formula C20H13Cl2N3O and a molecular weight of 382.24 g/mol.

The molecular framework of 1-Phenyl-1H-indole-2,3-dione 3-[N-(3,4-dichlorophenyl)hydrazone] (C20H13Cl2N3O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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