3'-Amino-6'-hydroxy-3h-spiro[2-benzofuran-1,9'-xanthen]-3-one CAS 3086-44-0

Description

The molecular framework of 3'-Amino-6'-hydroxy-3h-spiro[2-benzofuran-1,9'-xanthen]-3-one (C20H13NO4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

3'-Amino-6'-hydroxy-3h-spiro[2-benzofuran-1,9'-xanthen]-3-one (CAS 3086-44-0) is a high-purity analytical reference standard with the molecular formula C20H13NO4 and a molecular weight of 331.32 g/mol.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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