N'-Acetylacetohydrazide CAS 3148-73-0

Description

Compared with other compounds in its class, the molecular weight of 116.12 g/mol gives N'-Acetylacetohydrazide distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

N'-Acetylacetohydrazide (CAS 3148-73-0), molecular formula C4H8N2O2 and molecular weight 116.12 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of N'-Acetylacetohydrazide (C4H8N2O2) is characterized by an amino-acid-derived structure, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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