Ethyl 5-amino-3-(2-hydroxyethoxy)-1-phenyl-1H-pyrazole-4-carboxylate CAS 321391-76-8

Description

The molecular framework of Ethyl 5-amino-3-(2-hydroxyethoxy)-1-phenyl-1H-pyrazole-4-carboxylate (C14H17N3O4) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

Ethyl 5-amino-3-(2-hydroxyethoxy)-1-phenyl-1H-pyrazole-4-carboxylate (CAS 321391-76-8), molecular formula C14H17N3O4 and molecular weight 291.30 g/mol, is a CATO reference standard with documented purity and traceability.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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