1,3-Dioxo-2-(pyridin-4-yl)isoindoline-5-carboxylic acid CAS 328265-52-7

Description

Compared with other compounds in its class, the molecular weight of 316.31 g/mol gives 1,3-Dioxo-2-(pyridin-4-yl)isoindoline-5-carboxylic acid distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

1,3-Dioxo-2-(pyridin-4-yl)isoindoline-5-carboxylic acid (CAS 328265-52-7) is a high-purity analytical reference standard with the molecular formula C19H12N2O3 and a molecular weight of 316.31 g/mol.

The molecular framework of 1,3-Dioxo-2-(pyridin-4-yl)isoindoline-5-carboxylic acid (C19H12N2O3) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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