| CAS No. | 330177-51-0 |
| Molecular Formula | C13H20N4O3 |
| Molecular Weight | 280.32 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Compared with other compounds in its class, the molecular weight of 280.32 g/mol gives 2-((5-(3-Methylpiperazin-1-yl)-2-nitrophenyl)amino)ethanol distinctive chromatographic separation conditions and mass-spectrometry parameter settings.
CATO supplies 2-((5-(3-Methylpiperazin-1-yl)-2-nitrophenyl)amino)ethanol (CAS 330177-51-0), a certified reference material (molecular formula C13H20N4O3, MW 280.32 g/mol) for analytical method development and quality control.
The molecular framework of 2-((5-(3-Methylpiperazin-1-yl)-2-nitrophenyl)amino)ethanol (C13H20N4O3) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
| Molecular Formula | C13H20N4O3 |
| Molecular Weight | 280.32 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 6.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 4 |