(E)-4-((4-nitrobenzylidene)amino)phenyl 4-(tert-butyl)benzoate CAS 331460-54-9

Purity 97%+In StockPharmaceutical Standards
CAS No.331460-54-9
Molecular FormulaC24H22N2O4
Molecular Weight402.44 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

(E)-4-((4-nitrobenzylidene)amino)phenyl 4-(tert-butyl)benzoate CAS 331460-54-9 chemical structure

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including (E)-4-((4-nitrobenzylidene)amino)phenyl 4-(tert-butyl)benzoate.

(E)-4-((4-nitrobenzylidene)amino)phenyl 4-(tert-butyl)benzoate (CAS 331460-54-9), molecular formula C24H22N2O4 and molecular weight 402.44 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of (E)-4-((4-nitrobenzylidene)amino)phenyl 4-(tert-butyl)benzoate (C24H22N2O4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

CATO manufactures (E)-4-((4-nitrobenzylidene)amino)phenyl 4-(tert-butyl)benzoate under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.

Physical & Chemical Properties

Molecular FormulaC24H22N2O4
Molecular Weight402.44 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore in a cool place (≤25°C), protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)15.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)2025
Nitrogen Atoms2

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