4-{[(5-chloro-2-hydroxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one CAS 339020-79-0

Description

As a certified reference material (CRM), 4-{[(5-chloro-2-hydroxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

4-{[(5-chloro-2-hydroxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one (CAS 339020-79-0) is a high-purity analytical reference standard with the molecular formula C10H8ClN3O2 and a molecular weight of 237.64 g/mol.

The molecular framework of 4-{[(5-chloro-2-hydroxyphenyl)amino]methylidene}-4,5-dihydro-1H-pyrazol-5-one (C10H8ClN3O2) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

Physical & Chemical Properties

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