Ethyl 1-(4-nitrophenyl)-3a',4',8',8a'-tetrahydrospiro[piperidine-4,6'-[1,3]dioxepino[5,6-d]isoxazole]-3'-carboxylate CAS 339110-80-4

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

Ethyl 1-(4-nitrophenyl)-3a',4',8',8a'-tetrahydrospiro[piperidine-4,6'-[1,3]dioxepino[5,6-d]isoxazole]-3'-carboxylate (CAS 339110-80-4) is a high-purity analytical reference standard with the molecular formula C19H23N3O7 and a molecular weight of 405.40 g/mol.

Based on its molecular formula C19H23N3O7, Ethyl 1-(4-nitrophenyl)-3a',4',8',8a'-tetrahydrospiro[piperidine-4,6'-[1,3]dioxepino[5,6-d]isoxazole]-3'-carboxylate contains a flavonoid core, an amide group, a hydroxyl group, an ether linkage; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 405.40 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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