5-(4-Chlorobenzyl)-2,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one CAS 402570-41-6

Description

Under current pharmacopoeial and regulatory frameworks, quality-control requirements for this class of reference material explicitly involve the control of targets including 5-(4-Chlorobenzyl)-2,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one.

CATO supplies 5-(4-Chlorobenzyl)-2,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (CAS 402570-41-6), a certified reference material (molecular formula C12H9ClN4O, MW 260.68 g/mol) for analytical method development and quality control.

The molecular framework of 5-(4-Chlorobenzyl)-2,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one (C12H9ClN4O) is characterized by a nitrogen-containing heterocyclic scaffold, an amide group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 5-(4-Chlorobenzyl)-2,5-dihydro-4H-pyrazolo[3,4-d]pyrimidin-4-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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