2-Hydrazineyl-1-phenyl-1H-benzo[d]imidazole CAS 4502-99-2
| CAS No. | 4502-99-2 |
| Molecular Formula | C13H12N4 |
| Molecular Weight | 224.26 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The molecular framework of 2-Hydrazineyl-1-phenyl-1H-benzo[d]imidazole (C13H12N4) is characterized by a nitrogen-containing heterocyclic scaffold, a nitrile group. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
2-Hydrazineyl-1-phenyl-1H-benzo[d]imidazole (CAS 4502-99-2), molecular formula C13H12N4 and molecular weight 224.26 g/mol, is a CATO reference standard with documented purity and traceability.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.
Physical & Chemical Properties
| Molecular Formula | C13H12N4 |
| Molecular Weight | 224.26 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | Light-sensitive; avoid direct strong light |
| Degree of Unsaturation (DBE) | 10.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1958 |
| Nitrogen Atoms | 4 |
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Recommended Standards
- 2-Ethyl-4-isopropoxy-6-methoxypyrimidine-5-carbaldehyde (CAS 811802-52-5)
- 2-Hydrazinylidene-1,2-diphenylethan-1-one (CAS 5344-88-7)
- 2-Amino-5-benzyloxybenzonitrile (CAS 38711-02-3)
- 2-Methyl-6-phenyl-5H-pyrrolo[3,2-d]pyrimidin-4-amine (CAS 72549-61-2)
- (1R,4S)-4-methoxycyclopent-2-en-1-ol (CAS 194606-96-7)
- 2-Amino-3,5-dimethoxybenzoic acid (CAS 79263-00-6)
- 3-ethoxy-4-(methylamino)cyclobut-3-ene-1,2-dione (CAS 29950-13-8)
- 2-(4-Ethoxyphenyl)thiazole-5-carboxylic acid (CAS 886370-17-8)
- 3-(Aminomethyl)benzo[b]thiophene (CAS 40615-04-1)
- Spiro[3.6]decan-1-one (CAS 41597-00-6)
- tert-Butyl 2-(pyridin-2-yl)-6,7-dihydropyrazolo[1,5-a]pyrazine-5(4H)-carboxylate (CAS 1624262-00-5)
- (Z)-N'-Hydroxy-2-[2-(4-methoxyphenyl)-4-oxo-4H,5H-pyrazolo[1,5-a]pyrazin-5-yl]ethanimidamide (CAS 1255790-87-4)
- (E)-5-((Dimethylamino)methylene)-6,7-dihydrobenzo[b]thiophen-4(5h)-one (CAS 209741-01-5)
- Semaglutide Impurity 165 (CAS CATO-C4X-2226165)
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