| CAS No. | 477709-32-3 |
| Molecular Formula | C23H19N7O2S |
| Molecular Weight | 457.51 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
The molecular framework of 4-Methyl-3-((4-nitrobenzyl)thio)-5-(1-phenyl-5-(1H-pyrrol-1-yl)-1H-pyrazol-4-yl)-4H-1,2,4-triazole (C23H19N7O2S) is characterized by a steroid core, an amide group, a sulfone group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
4-Methyl-3-((4-nitrobenzyl)thio)-5-(1-phenyl-5-(1H-pyrrol-1-yl)-1H-pyrazol-4-yl)-4H-1,2,4-triazole (CAS 477709-32-3), molecular formula C23H19N7O2S and molecular weight 457.51 g/mol, is a CATO reference standard with documented purity and traceability.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), 4-Methyl-3-((4-nitrobenzyl)thio)-5-(1-phenyl-5-(1H-pyrrol-1-yl)-1H-pyrazol-4-yl)-4H-1,2,4-triazole meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C23H19N7O2S |
| Molecular Weight | 457.51 g/mol |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 18.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 7 |
| Sulfur Atoms | 1 |