1-(4-Bromophenyl)-3-(5-phenyl-1-(2,4,6-trichlorophenyl)-1H-pyrazol-4-yl)urea CAS 477713-91-0

Description

1-(4-Bromophenyl)-3-(5-phenyl-1-(2,4,6-trichlorophenyl)-1H-pyrazol-4-yl)urea (CAS 477713-91-0), molecular formula C22H14BrCl3N4O and molecular weight 536.64 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 1-(4-Bromophenyl)-3-(5-phenyl-1-(2,4,6-trichlorophenyl)-1H-pyrazol-4-yl)urea (C22H14BrCl3N4O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage, chlorine/bromine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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