1-(2,6-Dichlorobenzyl)-3-((4-fluorophenyl)imino)-5-methylindolin-2-one CAS 478043-06-0

Description

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

1-(2,6-Dichlorobenzyl)-3-((4-fluorophenyl)imino)-5-methylindolin-2-one (CAS 478043-06-0), molecular formula C22H15Cl2FN2O and molecular weight 413.27 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of 1-(2,6-Dichlorobenzyl)-3-((4-fluorophenyl)imino)-5-methylindolin-2-one (C22H15Cl2FN2O) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage, fluorine/chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by its high hydrophobicity (logP), which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Halogen substitution (F/Cl/Br/I) produces a distinctive isotope pattern in mass spectrometry, a valuable aid for qualitative confirmation.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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