(E)-3-(3,4-dimethylphenyl)-N'-(4-methoxybenzylidene)-4-oxo-3,4-dihydrophthalazine-1-carbohydrazide CAS 478045-74-8
| CAS No. | 478045-74-8 |
| Molecular Formula | C25H22N4O3 |
| Molecular Weight | 426.47 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
The occurrence of (E)-3-(3,4-dimethylphenyl)-N'-(4-methoxybenzylidene)-4-oxo-3,4-dihydrophthalazine-1-carbohydrazide is closely related to a steroid core, an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.
CATO supplies (E)-3-(3,4-dimethylphenyl)-N'-(4-methoxybenzylidene)-4-oxo-3,4-dihydrophthalazine-1-carbohydrazide (CAS 478045-74-8), a certified reference material (molecular formula C25H22N4O3, MW 426.47 g/mol) for analytical method development and quality control.
(E)-3-(3,4-dimethylphenyl)-N'-(4-methoxybenzylidene)-4-oxo-3,4-dihydrophthalazine-1-carbohydrazide (C25H22N4O3) contains a steroid core, an amide group, a hydroxyl group, an ether linkage. It is notably hygroscopic, the carboxyl group imparts weak acidity; these physicochemical properties determine the optimal preparation and storage of its standard solutions. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), (E)-3-(3,4-dimethylphenyl)-N'-(4-methoxybenzylidene)-4-oxo-3,4-dihydrophthalazine-1-carbohydrazide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
Physical & Chemical Properties
| Molecular Formula | C25H22N4O3 |
| Molecular Weight | 426.47 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store in a cool place (≤25°C), protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 17.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 4 |
Related E Standards
- N-(2,3-Dimethylphenyl)-2-((3-ethyl-4-oxo-3,4,5,6,7,8-hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-2-yl)thio)acetamide (CAS 618879-76-8)
- N-(2,6-Dimethylphenyl)-2-((3-ethyl-4-oxo-3,4,5,6,7,8-hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-2-yl)thio)acetamide (CAS 577996-82-8)
- N-(3,4-Dimethylphenyl)-2-((3-ethyl-4-oxo-3,4,5,6,7,8-hexahydrobenzo[4,5]thieno[2,3-d]pyrimidin-2-yl)thio)acetamide (CAS 618879-79-1)
- 2-((4-(N-(2,4-dimethylphenyl)sulfamoyl)phenyl)carbamoyl)benzoic acid (CAS 433310-45-3)
- 2-((4-(n-(3,4-Dimethylphenyl)sulfamoyl)phenyl)carbamoyl)benzoic acid (CAS 606924-86-1)
- 2-((4-(N-(3,5-dimethylphenyl)sulfamoyl)phenyl)carbamoyl)benzoic acid (CAS 433324-06-2)
- Ethyl 5-(2-(3,4-dimethylphenyl)-2-oxoethoxy)-2-phenylbenzofuran-3-carboxylate (CAS 6572-58-3)
- 1-(2,6-Dimethylphenyl)-3-((5-((2-fluorophenyl)amino)-1,3,4-thiadiazol-2-yl)thio)pyrrolidine-2,5-dione (CAS 742095-19-8)
Recommended Standards
- (3Z)-3-{[3-nitro-4-(4-phenylpiperazin-1-yl)phenyl]methylidene}-2,3-dihydro-1H-indol-2-one (CAS 866041-12-5)
- (R,R)-1,2-Bis[(2-methylphenyl)-(phenyl)phosphino]ethane (CAS 81157-90-6)
- L-Cysteine-Glutathione Disulfide (CAS 13081-14-6)
- (S,S)-1,2-Bis[(2-methylphenyl)-(phenyl)phosphino]ethane (CAS 810667-85-7)
- {[(5-methyl-3-nitro-1H-pyrazol-1-yl)methyl]thio}acetic acid (CAS 1020724-69-9)
- n-(1-Methoxypropan-2-yl)-1-methyl-1h-pyrazol-3-amine (CAS 1249785-08-7)
- Sodium 2-(4-Ethylphenyl)thiazole-4-acetate (CAS 1015533-53-5)
- Sodium 1-(2-pyridin-4-ylethyl)piperidine-4-carboxylate (CAS 1158260-61-7)
- 4-(4′-N,N-Dimethylaminophenyl)urazole (CAS 883455-55-8)
- 2-(2-Phenyl-1H-indol-3-yl)quinoline-4-carboxylic acid (CAS 89391-08-2)
- 1-(1-Propylpiperidin-3-yl)ethan-1-ol (CAS 1216130-24-3)
- tert-Butyl 6-methoxy-2-nitro-1H-indole-1-carboxylate (CAS 1881331-23-2)
- Methyl 2-(6-(trifluoromethyl)pyridin-3-yl)acetate (CAS 1363210-38-1)
- C-Type Natriuretic Peptide (32-53) (human, porcine, rat) (CAS 127869-51-6)
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