N'-(4-(Benzyloxy)benzylidene)-2,4-dihydroxybenzohydrazide CAS 478534-21-3

Purity 97%+In StockPharmaceutical Standards
CAS No.478534-21-3
Molecular FormulaC21H18N2O4
Molecular Weight362.38 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

N'-(4-(Benzyloxy)benzylidene)-2,4-dihydroxybenzohydrazide CAS 478534-21-3 chemical structure

Description

The occurrence of N'-(4-(Benzyloxy)benzylidene)-2,4-dihydroxybenzohydrazide is closely related to a flavonoid core, an amide group, a hydroxyl group, an ether linkage in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

N'-(4-(Benzyloxy)benzylidene)-2,4-dihydroxybenzohydrazide (CAS 478534-21-3), molecular formula C21H18N2O4 and molecular weight 362.38 g/mol, is a CATO reference standard with documented purity and traceability.

The molecular framework of N'-(4-(Benzyloxy)benzylidene)-2,4-dihydroxybenzohydrazide (C21H18N2O4) is characterized by a flavonoid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), N'-(4-(Benzyloxy)benzylidene)-2,4-dihydroxybenzohydrazide meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

Molecular FormulaC21H18N2O4
Molecular Weight362.38 g/mol
AppearancePale yellow to yellow crystalline powder
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening
Degree of Unsaturation (DBE)14.0
UV AbsorptionUV-absorbing
Structural FeaturesAromatic ring present
CAS Registry Year (est.)2025
Nitrogen Atoms2

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