| CAS No. | 481-20-9 |
| Molecular Formula | C27H48 |
| Molecular Weight | 372.67 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
5β-Cholestane (CAS 481-20-9), molecular formula C27H48 and molecular weight 372.67 g/mol, is a CATO reference standard with documented purity and traceability.
Based on its molecular formula C27H48, 5β-Cholestane contains an aromatic ring system; these features give the compound characteristic absorption bands in UV spectroscopy. The molecular weight of 372.67 g/mol is directly relevant to the identification of the molecular ion in mass spectrometry. The aromatic system may exhibit native fluorescence, enabling detection with a fluorescence detector (FLD).
As a certified reference material (CRM), 5β-Cholestane meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C27H48 |
| Molecular Weight | 372.67 g/mol |
| Appearance | Off-white to pale yellow crystalline powder |
| Purity | >90% |
| Storage | Store at room temperature |
| Solubility (reference) | Soluble in chloroform, dichloromethane, ethyl acetate |
| Stability | Stable under recommended storage conditions |
| Degree of Unsaturation (DBE) | 4.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 1957 |