6'-Amino-1-benzyl-3'-methyl-2-oxo-2'H-spiro[indoline-3,4'-pyrano[2,3-c]pyrazole]-5'-carbonitrile CAS 496805-22-2
| CAS No. | 496805-22-2 |
| Molecular Formula | C22H17N5O2 |
| Molecular Weight | 383.40 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
Chemical Structure
Description
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
CATO supplies 6'-Amino-1-benzyl-3'-methyl-2-oxo-2'H-spiro[indoline-3,4'-pyrano[2,3-c]pyrazole]-5'-carbonitrile (CAS 496805-22-2), a certified reference material (molecular formula C22H17N5O2, MW 383.40 g/mol) for analytical method development and quality control.
The molecular framework of 6'-Amino-1-benzyl-3'-methyl-2-oxo-2'H-spiro[indoline-3,4'-pyrano[2,3-c]pyrazole]-5'-carbonitrile (C22H17N5O2) is characterized by a steroid core, an amide group, a hydroxyl group, an ether linkage. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
CATO manufactures 6'-Amino-1-benzyl-3'-methyl-2-oxo-2'H-spiro[indoline-3,4'-pyrano[2,3-c]pyrazole]-5'-carbonitrile under ISO 17034:2016 accreditation with dual CNAS and ANAB accreditation. Each batch is characterized by orthogonal methods and supplied with a full Certificate of Analysis (COA), ensuring metrological traceability.
Physical & Chemical Properties
| Molecular Formula | C22H17N5O2 |
| Molecular Weight | 383.40 g/mol |
| Appearance | Pale yellow to yellow crystalline powder |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in DMSO, DMF, methanol |
| Stability | May oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening |
| Degree of Unsaturation (DBE) | 17.0 |
| UV Absorption | UV-absorbing |
| Structural Features | Aromatic ring present |
| CAS Registry Year (est.) | 2025 |
| Nitrogen Atoms | 5 |
Related Amino Standards
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Recommended Standards
- 6-(Carboxymethyl)-4-(thiophene-2-sulfonyl)-3,4-dihydro-2H-1,4-benzoxazine-2-carboxylic acid (CAS 866134-97-6)
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- 6-(4-Oxo-3-(m-tolyl)-3,4-dihydrophthalazin-1-yl)-2H-benzo[b][1,4]oxazin-3(4H)-one (CAS 861209-38-3)
- Dihydrozeatin 7-Glucoside (CAS 91599-03-0)
- 8-(tert-Butyl)-3-fluoroquinolin-2-ol (CAS 518070-27-4)
- 2-((((1-Isopropyl-1H-pyrazol-5-yl)methyl)amino)methyl)benzoic acid hydrochloride (CAS 1856089-57-0)
- 2-Bromo-N-(3-methoxyphenyl)butanamide (CAS 1243049-89-9)
- 4-Methylmorpholin-4-ium 3-cyano-6-(thiophen-2-yl)-5-(2,2,2-trifluoroacetyl)pyridine-2-thiolate (CAS 301842-71-7)
- 2-Nitro-benzoic acid tert-butyl ester (CAS 55666-41-6)
- 3,5-Difluoroisonicotinoyl Chloride (CAS 383363-42-6)
- Methyl 5-methyl-1h-pyrrolo[2,3-b]pyridine-2-carboxylate (CAS 1373228-85-3)
- 1-Bromo-3-methoxypropane (CAS 36865-41-5)
- 8-(Benzyloxy)-3-chloroimidazo[1,2-a]pyridine (CAS 1373350-40-3)
- Semaglutide Impurity 128 (CAS 2490269-78-6)
Why CATO?
- ISO 17034 accredited reference material producer (CNAS + ANAB dual accreditation)
- 130,000+ catalog items in stock, COA supplied with every batch
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- Technical support: +86 18665070610 / Nicole.chen@cato-chem.com