1H-Pyrazolo[4,3-b]pyridin-3(2H)-one CAS 51617-92-6

Description

Compared with other compounds in its class, the molecular weight of 135.12 g/mol gives 1H-Pyrazolo[4,3-b]pyridin-3(2H)-one distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

CATO supplies 1H-Pyrazolo[4,3-b]pyridin-3(2H)-one (CAS 51617-92-6), a certified reference material (molecular formula C6H5N3O, MW 135.12 g/mol) for analytical method development and quality control.

The molecular framework of 1H-Pyrazolo[4,3-b]pyridin-3(2H)-one (C6H5N3O) is characterized by an amide group, a hydroxyl group, an ether linkage, an aromatic ring system. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. The presence of an aromatic ring or conjugated system gives the compound characteristic absorption bands in the UV-Vis region, making it well suited to UV or diode-array detection (DAD).

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

As a certified reference material (CRM), 1H-Pyrazolo[4,3-b]pyridin-3(2H)-one meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.

Physical & Chemical Properties

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