4-Methyl-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine CAS 53645-73-1

Description

Compared with other compounds in its class, the molecular weight of 210.23 g/mol gives 4-Methyl-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

4-Methyl-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (CAS 53645-73-1), molecular formula C12H10N4 and molecular weight 210.23 g/mol, is a CATO reference standard with documented purity and traceability.

4-Methyl-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine (C12H10N4) contains a nitrogen-containing heterocyclic scaffold, a nitrile group. Its high carbon-to-hydrogen ratio confers good lipid solubility; these physicochemical properties determine the optimal preparation and storage of its standard solutions. The high carbon-to-hydrogen ratio imparts good lipophilicity, allowing liquid-liquid extraction with non-polar solvents such as n-hexane or ethyl acetate.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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