3,6-Bis(2-chloroethyl)piperazine-2,5-dione CAS 5394-22-9

Purity 97%+In StockPharmaceutical Standards
CAS No.5394-22-9
Molecular FormulaC8H12Cl2N2O2
Molecular Weight239.10 g/mol
CategoryPharmaceutical Standards / API Standards
AvailabilityIn Stock

Chemical Structure

3,6-Bis(2-chloroethyl)piperazine-2,5-dione CAS 5394-22-9 chemical structure

Description

Compared with other compounds in its class, the molecular weight of 239.10 g/mol gives 3,6-Bis(2-chloroethyl)piperazine-2,5-dione distinctive chromatographic separation conditions and mass-spectrometry parameter settings.

CATO supplies 3,6-Bis(2-chloroethyl)piperazine-2,5-dione (CAS 5394-22-9), a certified reference material (molecular formula C8H12Cl2N2O2, MW 239.10 g/mol) for analytical method development and quality control.

The molecular framework of 3,6-Bis(2-chloroethyl)piperazine-2,5-dione (C8H12Cl2N2O2) is characterized by an amide group, a carboxylic acid group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

Molecular FormulaC8H12Cl2N2O2
Molecular Weight239.10 g/mol
AppearanceWhite to pale yellow crystalline powder. (slight halide-like odor, faint amine-like odor, low volatility)
Purity97%+
StorageStore at 2-8°C, protect from light, keep sealed and dry, keep tightly closed to prevent evaporation
Solubility (reference)Soluble in DMSO, DMF, methanol
StabilityMay oxidize slowly on prolonged air exposure; Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions
Degree of Unsaturation (DBE)3.0
UV AbsorptionUV-absorbing
CAS Registry Year (est.)1958
Halogen ContentCl-containing
Nitrogen Atoms2

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