| CAS No. | 53948-46-2 |
| Molecular Formula | C8H18Cl2N5O3P |
| Molecular Weight | 334.14 g/mol |
| Category | Pharmaceutical Standards / API Standards |
| Availability | In Stock |
The occurrence of Hydroxy cyclophosphamide Semicarbazone is closely related to an amide group, a phosphonate group, a carboxylic acid group, a hydroxyl group, an ether linkage, chlorine substitution in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.
Hydroxy cyclophosphamide Semicarbazone (CAS 53948-46-2) is a high-purity analytical reference standard with the molecular formula C8H18Cl2N5O3P and a molecular weight of 334.14 g/mol.
The molecular framework of Hydroxy cyclophosphamide Semicarbazone (C8H18Cl2N5O3P) is characterized by an amide group, a phosphonate group, a carboxylic acid group, a hydroxyl group, an ether linkage, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.
This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.
As a certified reference material (CRM), Hydroxy cyclophosphamide Semicarbazone meets the metrological traceability requirements of ISO/IEC 17025 laboratories. Batch-to-batch consistency is monitored via control charts to guarantee comparable assigned values across lots.
| Molecular Formula | C8H18Cl2N5O3P |
| Molecular Weight | 334.14 g/mol |
| Purity | 97%+ |
| Storage | Store at 2-8°C, protect from light, keep sealed and dry |
| Solubility (reference) | Soluble in methanol, ethanol, DMSO |
| Stability | Light-sensitive; avoid direct strong light; Hygroscopic; reseal immediately after opening; Contains ester bond; hydrolyzes under strong alkaline conditions |
| Degree of Unsaturation (DBE) | 1.5 |
| CAS Registry Year (est.) | 1972 |
| Halogen Content | Cl-containing |
| Nitrogen Atoms | 5 |