7-chloro-3-methyl-1H-pyrazolo[4,3-d]pyrimidine CAS 5399-95-1

Description

The occurrence of 7-chloro-3-methyl-1H-pyrazolo[4,3-d]pyrimidine is closely related to a nitrile group, chlorine substitution in its molecular formula, which determines its behaviour during synthesis, purification, or degradation.

7-chloro-3-methyl-1H-pyrazolo[4,3-d]pyrimidine (CAS 5399-95-1) is a high-purity analytical reference standard with the molecular formula C6H13ClN4 and a molecular weight of 176.65 g/mol.

The molecular framework of 7-chloro-3-methyl-1H-pyrazolo[4,3-d]pyrimidine (C6H13ClN4) is characterized by a nitrile group, chlorine substitution. In reversed-phase chromatography, its retention is governed primarily by the protonation state of its nitrogen-containing groups, which provides a practical basis for HPLC-UV/DAD or LC-MS method development. Nitrogen-containing functional groups confer excellent ionization efficiency in positive-ion electrospray (ESI) mode, making the compound well suited to LC-MS/MS analysis.

This reference standard is suitable for quantitative analysis on major chromatographic platforms including HPLC, GC, LC-MS/MS, and GC-MS, serving as an external calibration standard or a system suitability test reference material.

The assigned purity is verified by mass-balance and quantitative NMR (qNMR) approaches, with the expanded uncertainty reported at a 95% confidence level (k=2). Batch homogeneity and stability are independently assessed for every lot.

Physical & Chemical Properties

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